Validating affymetrix chip results with real time pcr who is drake dating now 2016
Such molecular phenotypes defined pre-clinically may ultimately impact on the evaluation of a particular drug in a living cell.
Dissecting the specific effect of a drug on a defined biological process is often complicated by the plethora of secondary effects that arise during extended exposure.
The aim of these pharmacogenomic approaches is to provide new information on how drugs can impact on the translational read-out within a defined cellular background.
We describe an approach that permits the analysis of de-novo m RNA-ribosome association in-vivo during short drug exposures.
For polysome analysis, cells in the growing phase (60% confluence) were hypertonically shocked by shifting to medium containing 300 m M Na Cl for 50 min.Rapamycin and DMSO were kept on the cells throughout the 30 mins recovery period (total time of exposure to rapamycin was 50 mins).